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In vivo, LA exists as free and protein-bound (predominant form) and is formed by the substitution of sulfur groups for hydrogen at positions 6 and 8 of octane groups [26]

Endotoxin levels are determined by measuring the activity of Factor C in the presence of the colorless chromogenic synthetic peptide substrate (Ac-Ile-Glu-Ala-Arg-pNA) that releases p-nitroaniline (pNA) after proteolysis, producing a yellow color that can be measured at an absorbance of 405 nm

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For this, we used siRNA technology to deplete RECQ1, MUS81, LIG4 or ELL in U2OS cells transduced with the HRASV12 or cyclin E1 retroviral vectors