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Applied Sciences , 10 , 2065

Biology 12(2):280 Sankarapandian V, Venmathi Maran BA, Rajendran RL, Jogalekar MP, Gurunagarajan S, Krishnamoorthy R, Gangadaran P, Ahn BC (2022) An update on the effectiveness of probiotics in the prevention and treatment of cancer

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Immunohistochemistry Mice were perfused with ice-cold PBS, followed by Zambonis fixative

This critical step prevents condensation from forming inside the vial when bacteriostatic water is added, which can: Introduce uncontrolled water into the reconstitution (altering final concentration) Create localized temperature differentials affecting peptide stability Complicate visual verification of complete reconstitution Step 3 Sterilize the Vial Top and Prepare Bacteriostatic Water Using an alcohol prep pad, thoroughly swab the rubber stopper of both the peptide vial and bacteriostatic water vial

Zinnurova, A