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The copper(II) complex adds one copper ion (atomic mass approximately 64 daltons) plus typically a coordinated water or hydroxide molecule, depending on conditions, giving the complex an overall mass in the range of approximately 400-420 daltons

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Two mutant complexes, TRX(C35A)TTXNIP(C120S/C170S/C205S/C267S) (hereafter referred to as Com1) and TRX(C35A)TTXNIP(C170S/C205S/C267S) (hereafter referred to as Com2), were crystallized to X-ray diffraction quality and their structures were determined using the multiple anomalous wavelength dispersion method, together with the molecular replacement (MR) method

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