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For each sample, 120 L of plasma was aliquoted into two round-bottom 96-well plates, kept on dry ice, sealed with plastic film, and stored at 80 C until LC-MS/MS sample preparation and analysis

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Additionally, neuroinflammation-driven oxidative stress damages monoaminergic neurons in the raphe nuclei, disrupting serotonin homeostasis and further destabilizing REM sleep [34] (Figure 1)

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