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For single sgRNA cloning, the oligonucleotides containing 20 bp protospacer and overhang were obtained from Integrated DNA Technologies and cloned into the Bsm BI-v2 (New England Biolabs, catalogue no

10.13863/j.issn1001-A4454.2010.09.018 80 GeorgeK

doi: 10.1556/APhysiol.93.2006.4.11 61 SmithLBWalkerWH

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elegans is associated with piRNAs, 22G-RNAs, and histone H3K9me3 and H3K27me3 marks 11,35,86,114

In the case of cancer, this process has a dual activity, since it is capable of promoting the survival of cancer cells, through protective autophagy, or participating in the death of these cells, inducing cytotoxic autophagy (116, 117)